Centre for Applied Molecular Biology (CAMB)

Health Sciences

In order to build a National capability in the newly emerging science, the Federal Ministry of Science & Technology (MoST) approved the establishment of Centre for Applied Molecular Biology (CAMB) at University of the Punjab Lahore in 1987 which is located back to back with the laboratory block....

About Us

Vision, Mission, PEOs, and PLOs

Vision & Mission:
By acquiring and propagating world class education we strive to build an institution of learning where we seek to have a futuristic approach in imparting knowledge, skills and confidence in students and to inoculate in them such critical abilities, wisdom and values which are necessary for a forward looking coherent civil society where knowledge is disseminated and service rendered with the highest sense of moral uprightness and social responsibility to the nation, region and the world at large. A culture of excellence and continuous improvement is our hallmark.
Goals / Objectives:
The centre is functioning with the following main objectives:
Goal oriented molecular biological research on particular concerns related to economic needs of the country for agriculture, health and industry.
Generate a cadre of manpower through teaching and training, specifically trained in the field of molecular biology and forensic science to cope with changing needs of the country.
Provide state of the art analytical services including forensic DNA analysis for crime investigation, molecular diagnosis of most prevalent infectious diseases and DNA sequencing to extend support to the community.
Conduct symposia, seminars, workshops in the frontiers of Molecular Biological research and forensic science.
Major Achievements:
Goal Oriented Research Activities:
A cadre of skilled scientists and students are working on important issues in health and agriculture. Following are few main outcomes of the applied research.
i.
PCR based diagnostic procedures were developed which are being used in the diagnosis of different infectious diseases
ii.
In order to cope with the changing needs of crime investigation, Forensic DNA typing procedures were developed which are being used for DNA analysis in crime investigation.
iii.
CAMB has developed genetically modified lines of sugarcane (CAMB-1, CAMB-2 & CAMB-3) with disease resistance against Sugarcane Mosaic Virus (SCMV). These genetically modified plant lines are more tolerant to diseases, specifically viral diseases, and have very good vigor in Pakistan's natural environment.
iv
Asthma
Public Service Activities:
i.
The centre has provided services for DNA analysis to the Law enforcement agencies & Courts in more than 3500 cases including rape, murder, sodomy, dead body identification, bomb blasts, robbery and paternity.
ii.
The centre has provided diagnostic services to the general public in about 200,000 tests including HCV Qualitative, HCV Quantitative, HCV genotyping, HBV Qualitative and HBV Quantitative. The centre has also provided services free of cost for testing 40,225 tests under “Prime Minister Program (PMP)” for Hepatitis.
iii.
The core facility of CAMB for DNA sequencing and genotyping and is well equipped with state of the art equipment and the biggest DNA analysis facility in Pakistan. The laboratory has provided services to the researchers of different organizations for DNA sequencing and genotyping in approximate 400,000 samples.
Academic Programs:
After becoming part of university of Punjab in July 2015, CAMB enrolled its first batch of M. Phil and Ph.D, currently, 54 M. Phil and 16 Ph.D students are enrolled at CAMB for perusing their degrees.

In order to build a National capability in the newly emerging science, the Federal Ministry of Science & Technology (MoST) approved the establishment of Centre for Applied Molecular Biology (CAMB) at University of the Punjab Lahore in 1987 which is located back to back with the laboratory block of the Centre of Excellence in Molecular Biology (CEMB). In March 2015, the Prime Minister of Pakistan approved the transfer of CAMB to University of the Punjab. Accordingly, MoST handed over the administrative control of CAMB to University of the Punjab, Lahore w.e.f 01.07.2015 along with all assets and liabilities.
The CAMB’s laboratory block includes Forensic DNA Typing, Molecular Diagnostics of infectious diseases, proteomics, virology and DNA Core Facility laboratories and is surrounded by a vast area of land where crops and other plants are sown for research in agriculture molecular biology. CAMB being the renowned scientific research entity encourages the osmosis of research from Lab into different sectors of the society. It strives to provide a suitable environment and encourages researchers to work on collaborative research projects to advance knowledge, to propose solutions to some of the major issues of today and to develop new technologies for the future.
CAMB is providing reliable, accurate, rapid and more economical diagnostic & Forensic services to the law enforcement agencies, courts and general public and DNA Sequencing and genotyping facilities to the various academic & R&D organization throughout Pakistan. Centre is also offering M. Phil & Ph.D degree programs in “Molecular Biology & Forensic sciences” in academics. Students of various universities also use the platform of CAMB for completion of their degrees. The whole set up of CAMB represents a unique and most economical mode to optimally utilize the extremely limited resources of personnel and materials.
5 Days Hands-On Training Workshop (DNA Sequencing) 2023

Programs

Presently the Centre for Applied Molecular Biology (CAMB) offer a wide range of degree programs.

Our Staff

  • All Staff
  • Teaching
  • Administrative
Prof. Dr. Rehan Sadiq Shaikh

Prof. Dr. Rehan Sadiq Shaikh

Professor & Director

Dr. Aleena Sumrin

Dr. Aleena Sumrin

Associate Professor

Dr. Muhammad Asif Naeem

Dr. Muhammad Asif Naeem

Associate Professor

Dr. Muhammad Zafar Saleem

Dr. Muhammad Zafar Saleem

Assistant Professor

Dr. Manzoor Hussain

Dr. Manzoor Hussain

Assistant Professor

Dr. Muhammad Bilal

Dr. Muhammad Bilal

Assistant Professor

Dr. Hamid Bashir

Dr. Hamid Bashir

Assistant Professor

Dr. Nazim Hussain

Dr. Nazim Hussain

Assistant Professor

Dr. Safdar Hussain

Dr. Safdar Hussain

Assistant Professor

Dr. Rukhsana Parveen

Dr. Rukhsana Parveen

Assistant Professor

Dr. Ghulam Zahara Jahangir

Dr. Ghulam Zahara Jahangir

Assistant Professor

Dr. Muhammad Usman Ghani

Dr. Muhammad Usman Ghani

Assistant Professor

Dr. Muhammad Adnan Shan

Dr. Muhammad Adnan Shan

Assistant Professor

Dr. Farheena Iqbal Awan

Dr. Farheena Iqbal Awan

Assistant Professor

Dr. Saima Younas

Dr. Saima Younas

Assistant Professor

Dr. Muhammad Shahzad

Dr. Muhammad Shahzad

Assistant Professor

Mr. Muhammad Akram

Mr. Muhammad Akram

Research Officer cum Lecturer

Ms. Sadia Manzoor

Ms. Sadia Manzoor

Research Officer cum Lecturer

Mr. Fiaz Ahmad

Mr. Fiaz Ahmad


Incharge IT & RML CAMB

Centre for Applied Molecular Biology (CAMB)
Health Sciences

In order to build a National capability in the newly emerging science, the Federal Ministry of Science & Technology (MoST) approved the establishment of Centre for Applied Molecular Biology (CAMB) at University of the Punjab Lahore in 1987 which is located back to back with the laboratory block of the Centre of Exc....

Last Three Year Articles



Year:
2015

1-
Butt AM, Castellano L, Idrees M.,; Next generation sequencing reveals differential expression profiles of hepatic microRNAs in chronic hepatitis C patients., Journal of Hepatology, 62, 2015
2-
Butt AM, Castellano L, Idrees M, Tong Y,; Deep sequencing shows microRNA expression in hepatitis C patients is associated with the end treatment response., Journal of Clinical Virology, 69, 2015
3-
Amat-ur-Rasool H, Saghir A, Idrees M,; Computational Prediction and Analysis of Envelop Glycoprotein Epitopes of DENV-2 and DENV-3 Pakistani isolates: A First Step towards Dengue Vaccine Development., PLOS ONE., 16, 2015
4-
Khubaib B, Idrees M, Afzal S, Wasim M.,; The genotype CC of IL-28B SNP rs12979860 is significantly associated with a sustained virological response in chronic HCV Pakistani patients., Journal of Digestive Diseases, 16, 2015
5-
Ali A, Idrees M.,; Expression of Hepatitis C Virus Core and E2 antigenic recombinant proteins and their use for development of diagnostic assays., International Journal of Infectious Diseases, 84, 2015
6-
Hussain M, Idrees M, Afzal S.,; Development of Global Consensus of Dengue Virus Envelope Glycoprotein for Epitopes based vaccine design., , 11(1), 2015
7-
Hussain A, Idrees M, Asif M, Ali L, Rasool M.,; Phylogenetic and 2D/3D Analysis of HCV 1a NS4A Gene/Protein in, Pakistani Isolates., Hepatitis Monthly, 15(6), 2015
8-
Mariam Shahid, Muhammad Farooq Sabar, Iqbal Bano, Ziaur Rahman, Zafar Iqbal, Sayyed Saadia Fatim Ali, Muhammad Ghani, Muhammad Iqbal & Tayyab Husnain,; (2015). Sequence variants on 17q21 are associated with the susceptibility of asthma in the population of Lahore, Pakistan., Journal of Asthma, 52, 2015
9-
Kashif Rehman, Muhammad Akram Tariq, Muhammad Farooq Sabar.,; (2015) Allele frequency distribution of CYP2C19*2 allelic variants associated with clopidogrel resistance in cardiac patients of Pakistan., Experimental and Therapeutic Medicine, 10, 2015
10-
Mariam Shahid, Muhammad Farooq Sabar, Ziaur Rahman, Iqbal Bano, Muhammad Usman Ghani, Samra Kousar, Muhammad Akram, Tayyab Husnain,; Urbanization triggers asthma in ‘C’ allele carriers for rs12603332, European Academy of Allergy and Clinical Immunology (EAACI), P04, Istanbul, Turkey, , 2015
11-
Javed Iqbal Wattoo, Muhammad Shahzad Iqbal, Muhammad Arif, Zafar Saleem, Muhammad Naveed Shahid and Muhammad Iqbal,; Homology Modeling, Functional Annotation and Comparative Genome Analysis of GBSS Enzyme in Rice and Maize Genomes, INTERNATIONAL JOURNAL OF AGRICULTURE & BIOLOGY, , 2015
12-
Muhammad Bilal, Muhammad Saeed, Idrees Ahmad Nasir, Bushra Tabassum, Mariam Zameer, Anwar Khan, Muhammad Tariq, Muhammad Aslam Javed, and Tayyab Husnain..,; Association mapping of cane weight and tillers per plant in sugarcane, Biotechnology & Biotechnological Equipment, 29, 2015
13-
Hamid Bashir ,; Simple procedure applying lactose induction and one step purification for high yield production of rh cifn, Biotechnology and Applied Biochemistry, , 2015
14-
Mariam Shahid, Muhammad Farooq Sabar, Iqbal Bano, Ziaur Rahman, Zafar Iqbal, Sayyed Saadia Fatim Ali, Muhammad Ghani, Muhammad Iqbal & Tayyab Husnain,; (2015). Sequence variants on 17q21 are associated with the susceptibility of asthma in the population of Lahore, Pakistan., Journal of Asthma, 52, 2015
15-
Mariam Shahid, Muhammad Farooq Sabar, Ziaur Rahman, Iqbal Bano, Muhammad Usman Ghani, Samra Kousar, Muhammad Akram, Tayyab Husnain,; Urbanization triggers asthma in ‘C’ allele carriers for rs12603332, European Academy of Allergy and Clinical Immunology (EAACI), P04, Istanbul, Turkey, , 2015
16-
Shan, M. A., Tahira, F., Shafique, M., Hussain, M., Perveen, R., Shahzad, M., Ehsan, F., Shahzad, M. S., Rehman, Z., Ali, Q., and Iqbal, M,; Estimation of different Biochemical Intensities in Drinking water from Easterm Region of Lahore City, International journal of Adv.Life.Sci, 2(3), 2015
17-
Muhammad Shafique Manzoor Hussain Muhamamd Adnan Shan Muhammad Shahzad Safdar Hussain Nazim Hussain Farhana Ehsan Rukhsana Perveen Muhammad Iqbal,; Genetic Diversity of 15 autosomal STR loci in the population of Southern Punjab Pakistan, Forensic Science International:Genetics, , 2015
18-
Ahmed N1, Bashir H, Zafar AU, Khan MA, Tahir S, Khan F, Khan MI, Akram M, Husnain T.,; Optimization of conditions for high-level expression and purification of human recombinant consensus interferon (rh-cIFN) and its characterization, Biotechnology & Applied Biochemistry, , 2015
19-
Muhammad Shafique, Manzoor Hussain, Muhamamd Adnan Shan, Muhammad Shahzad, Safdar Hussain, Nazim Hussain, Farhana Ehsan, Rukhsana Perveen, Muhammad Iqbal,; Genetic Diversity of 15 autosomal STR loci in the population of Southern Punjab Pakistan, Forensic Science International: Genetics, , 2015
20-
Nasrullah I, Butt AM, Tahir S, Idrees M, Tong Y,; Genomic analysis of codon usage show influence of mutation pressure, natural selection, and host features on Marburg virus evolution, BMC Evolutionary Biology, 15, 2015
21-
Akram M, Fatima Z, Purdy MA, Sue A, Saleem S, Amin I, Shahid M, Nawaz R, Idrees M,; Introduction and evolution of dengue virus type 2 in Pakistan: a phylogeographic analysis, Virology Journal, 12, 2015
22-
Shaheen MA, Idrees M,; Evidence-based consensus on the diagnosis, prevention and management of hepatitis C virus disease, World J Hepatol, 7, 2015
23-
Sabar, M.F., Ghani, M.U., Shahid, M., Sumrin, A., Ali, A., Akram, M., Tariq, M.A., Bano, I.,; Analysis of genetic variants of ADAM33 gene for Susceptibility of Asthma in Pakistan, Journal of Asthma, 10, 2015
24-
Iqbal Z., Akram A.M., Akhtar T., Khalid M., Aziz Z., Aleem A., Gill A.T., Khalid Am., Alanazi A., Shah I.H., Khalid M., Sabar M.F., and Iqbal M,; High Frequencies of Compound BCR-ABL Mutations and Their association with Imatinib Resistant, Disease Progression and Late Crhonic Phase Disease in Pakistani Chronic Myeloid Leukemia Patients Necessitate the Inclusion of Molecular Testing in Routine Clini, Blood Online Publication, 126, 2015
25-
Iqbal, Z., Akhtar T., Awan, T., Aleem, A., Sabir, N., Absar, A., Sabir, N., Absar M., Shammas, M.A., SHAH, I.H., Khalid, M., Taj, A.S,., Jameel, A., Alanazi, A., Gill, A.T., Hashmi, J.A., Hussain, A., Sabar, M.F., Khalid, A.M., Qazi M.,h., Karim, S., Sidd,; High Frequesy and poor prognosis of late childhood BCR-ABL positive and MLL-AF4 positive all define the need for advanced molecular diagnostics and improved therapeutic strateggies in pediatric B-ALL in Pakistan, Molecular Diagnosis & Therapy, 19, 2015
26-
H. Sadia, S. Manzoor, A. Wajid, M. Tayyab, S, Firyal, A.S. Hashmi, T. Yaqub, Z.U. Mughal, A.K, Mehmood, A.R. Awan, K. Mahmood, M.Y. Khan and M. Wasim,; COX-2 and BRCA 1 have altered expression profile in different canine tumors, Journal fo Plant and Animal Sciences, 26, 2015
27-
Haleema Sadia, Saba Manzoor, Abdul Wajid, Muhammat Tayyab, Sehrish Faryal, Abu Saeed Hashmi, Zia Ullah Mughal, Asim Khalid Mahmood, Tahir Yaqoob, Ali Raza Awan and Muhammad Wasim,; Feline mammary tumors show altered COX-2 and BRCA1 expression, Pakistan Journal of Zoology, 47, 2015
28-
Muhammad Hassan Siddiqi, Tanveer Akhtar, Allah Rakha, Ghazanfar Abbas, Akram Ali, Naeem Haider, Azam Ali, Sikdandar Hayat, Syeda Masooma, Jamil Ahmad, Muhammad Akram Tariq, Mannis Van Oven and Fazle Majid Khan,; Genetic characterization of the Makrani people of Pakistan from mitochondrial DNA control-region data, Legal Medicine, 134, 2015
29-
Sikandar Hayat, Tanveer Akhtar, Muhammad Hassan Siddiqi, Allah Rakha, Naeem Haider, Muhammad Tayyab, Chazanfar Abbas, Azam Ali, Syed Yasir Abbas Bukhari, Muhammad Akram Tariq and Fazle Majid Khan,; Mitochondrial DNA control region sequences study in Saraiki Population from Pakistan, Legal Medicine, 140, 2015
30-
A Khan, B Tabassum, I A Nasir, M Bilal, M Tariq, T Husnain,; Potato virus x from Pakistan: Coat protien sequence analysis, Journal of Animal and Plant Sciences, 25, 2015
31-
Hamid Bashir, Nadeem Ahmed, Mohsin Ahmad Khan, Ahmad Usman Zafar, Saad Tahir, Hina Kanwal, Faidad Khan, Zia ur Rahman and Tayyab Husnain,; Evaluating the auto induction expression system and one step purification for high level expression and purification of gallbladder derived rhIL 1 Ra, , , 2015
32-
Ruqeyah Abdul Majeed, Ahmad Ali ShAHID, Muhammad Arif Zafar Saleem Muhammad Naveed Shahid and Muhammad Iqbal,; First Report of Curvularia LUNATA Casuring Brown Leaf spots of Rice in Punjab, Pakistan, The American Phytopathological Society, 91, 2015
33-
Muhammad Usman Mirza, Noor-ul-Huda Ghori, Nazia Ikram, Abdur Rehman Adil and Sadia Manzoor,; Pharmacoinformatics approach for investigation of alternative potential hepatitis C Virus nonstructural protein 5 B inhibitors, Drug Design, Development and Therapy, 1825, 2015



Publications:
For the year 2015-16
International
=
45
National
=
14
Impact Factor
=
141.876
Trainings, Symposia, Exhibitions & Workshops:
CAMB has conducted series of training workshops for the police officers of various ranks, medico-legal doctors and for Judiciary and lawyers to promote the use of DNA technology in crime investigation.
Series of lectures were delivered in multiple training courses held at “Counter Terrorism Department (CTD), Chung, Lahore to train the police officers for procuring biological evidence in different cases for DNA analysis.
CAMB is always a key participant of the “Invention & Innovation Summit” organized by Pakistan Science Foundation (PSF). CAMB was declared as winner of the Invention & Innovation Summit-2012 & 2016 and was awarded Winning Shields.
CAMB organized number of training workshops for the researchers / officials of different organization including
 
a.
Two weeks hands on training on “samples preparation techniques for capillary electrophoresis, Analysis of Genotyping Data and interpretation of data for parentage verification of bulls” was provided to the 03 officials of Livestock and Dairy Development Department, Punjab
 
b.
One week hands on training on “Forensic DNA Analysis for Human Identification” was provided to the 06 officials of Forensic Medicine Department of Bannu Medical College, KPK including Prof. Dr. Muhammad Humanyun, Head of Department of Forensic Medicine, BMC
 
c.
Two weeks training on Forensic DNA Analysis was provided to Ms. Rabia Sultan, Research Associate, HEJ Karachi
Four-days” Hands on Training Workshop on Clinical Diagnostics (Oct 18-21, 2016)” jointly organized by Institute of Biochemistry & Biotechnology (IBB) and Centre for Applied Molecular Biology (CAMB), university of the Punjab Lahore
Series of seminars were organized at CAMB on different important aspects including advancements in Molecular Biology; New Technological tools and applications in biological research; Advances in Nucleic Acid Technology; Lungs and Bronchial Diseases etc.
CAMB participated in number scientific exhibitions to present to present its products and services including DICE-2013, distinguished innovations, collaborations and Entrepreneurship held at Gujrat University; Punjab Youth Festival 2014, held at International Expo Centre Lahore; S&T exhibition 2015 organized at PCSIR Lahore; Pak-China Business Forum (PCBF-2016) held at Expo Center Lahore; Trade promotion and industrial Development Conference & Exhibition (TPIDCE-2016)” held at Sheikhupura Chmaber of Commerece and Industry etc.
CAMB representation was made in the two day international “DNA Strategy Symposium” jointly organized by British High Commission of Pakistan, Ireland and Punjab Forensic Science Agency (PFSA) held at Pearl Continental Hotel, Lahore on January 18-19, 2016
CAMB made active participation in the 11th Biennial conference of Pakistan Society for Biochemistry and Molecular Biology held at Punjab University November.
CAMB DNA expert was invited as speaker in the seminar on “Forensic DNA Typing: Role of Judiciary and Investigating Agencies” held at Liaquat University of Medical & Health Sciences (LUMHS), Jamshoro on August 27, 2016. The seminar was chaired by Honourable Mr. Justice Amir Hani Muslim (Honurable judge of Supreme Court of Pakistan).
 

Conference Proceedings



Year:
2015

1-
Conference proceeding on Urbanization triggers asthma in ‘C’ allele carriers for rs12603332, European Academy of Allergy and Clinical Immunology (EAACI), P04, Istanbul, Turkey, , 2015
2-
Conference proceeding on Urbanization triggers asthma in ‘C’ allele carriers for rs12603332, European Academy of Allergy and Clinical Immunology (EAACI), P04, Istanbul, Turkey, , 2015



Will be updated soon

This Center, since its inception, has been providing the best available facilities to its researchers and scholars. Currently CAMB is one of the focal points of health, forensic sciences and agricultural research and education in the country. The CAMB laboratories along with more than 50 acres of farm land are devoted to researchers and students. The Laboratory Complex of CAMB with a covered area of 7000 square meters including a Laboratory Block, a Teaching Block and a Hostel for Ph.D. Research Scholars. The Laboratory Block is divided into four separate research units comprising a total of 40 Research Labs and four Conference Rooms; one Production Unit ,and one Support Facilities Unit comprising a Lab-aid Section (for washing, autoclaving and media preparation), an Animal House, an Insectary, six large Plant Growth Rooms, and storage space for research materials. The Teaching Block consists of a well equipped Library, Seminar Hall, Photocopy, Photography, Computer Rooms, a Conference Hall, Director's Office and Administration and Accounts section. There is a self-service canteen, a dining hall and 50 rooms in the research scholars' hostels. With all these facilities, CAMB offers the best environment for enthusiastic researchers/students who are eager to excel in their studies, outclass in their career and outshine in their lives.
Services

1) DNA Core Facility

This facility is providing following services to the researchers in the centre as well as other institutes of the country and abroad.
1.
DNA Sequencing >500bp/sample
2.
DNA Fragment Analysis 5-Dyes in a sample
3.
UV/VIS Spectroscopy/ELISA Reading
In near future we will also be able to provide the facilities of RNA/DNA extraction, DNA Amplification Purifications procedures.
 
Introduction
CAMB DNA Core Facility
CAMB has many unique features in the field of biotechnology and molecular biology research in Pakistan and among them DNA Core Facility has been the pride of the institute. It is one of the best facilities not only in Pakistan but most of the Asian countries. There are two components of the facility; DNA Sequencing and DNA Genotyping. The main objective of the facility is to provide a quality DNA analysis services to the researchers in CAMB as well as other research organizations and universities throughout the country. Other than this the facility also provides a technical help to the institutions regarding problems in this field.
DNA Sequencing/Genotyping Facility
Considering the upcoming demands and needs of DNA sequencing and genotyping in the field of biotechnology, CAMB initiated automated DNA sequencing, in 1999, on a single capillary 310 ABI-Genetic analyzer. Recognizing the significance of DNA sequencing in the molecular biology research, the lab was strengthened by adding four more 16-capillary, and one 48-capillary DNA Sequencers. So at this time DNA Sequencing facility of the centre is the largest sequencing facility in Pakistan with 5- DNA Analyzers from ABI with a total output of processing 112 samples at a time and thousands of samples daily. So it is the first such kind of high throughput sequencing/genotyping laboratory in Pakistan. The centre has produced many publications on the basis of data generated from this facility.
The data generated in this facility is accurate precise and reliable and can be compared with any other internationally well recognized labs. Researchers have a confidence on the quality of our services.
The success of the facility can be evidenced from the fact that many departments of the University of Punjab as well as other universities of the country have started their research programs considering that they would be able to avail the facility of analysis of their DNA samples accurately with good quality data generated from here.
While the facility is being sponsored by HEC to serve clients at the public sector universities (other than PU), it may eventually offer contract based services for public and private laboratories outside the universities. Currently we are rendering the services of DNA genotyping analysis of cattle to “Breed Improvement, Livestock and Dairy Development” department of Government of Punjab under a contract signed between the two organizations.
So, the DNA Core Facility is considered to be the key facility for DNA researchers in Pakistan. In the progress that CAMB has made and still making history in the field of Molecular Biology and Biotechnology, this facility has the major role
UV/VIS Spectroscopy/ELISA Reading
We can help you to analyze your samples on a spectrophotometer using the cuvettes or 96-well plates. We have a state of the art UV/VIS spectrophotometer i.e., SpectraMax-384 system from Molecular Devices USA. It can analyze single sample in a cuvette or up to 96 samples in a plate. It gives high quality analysis using very low volume samples.
Research and Development
The facility is also involved in genomics studies and the molecular biology research on genetic diseases. Currently, genetic basis of asthma disease is being investigated and study on the association of genomic variants with the disease in Pakistani population is being conducted. We have already found some polymorphic variants associated with the disease. MS, MPhil and PhD theses have been completed in this research and research articles have been published in international journals.
Quality of Services
DNA Sequencing
DNA CORE FACILITY provides automated Sanger sequencing using capillary-based ABI-3730, ABI-3130xl and ABI-3100 DNA Analyzers. If the sample is highly purified and accurately quantities, we guarantee more than 800bp sequence/sample with more than 99% accuracy. Our rates are comparable to international service providers especially for the high throughput samples and the quality of sequencing data generated here is beyond the doubts.
 
DNA Genotyping
DNA CORE FACILITY provides high quality and high throughput genotyping facility on the basis of DNA microsatellite and SNP analysis. CAMB researchers are very well experienced in STR and SNP based genotyping. We are always available for troubleshooting your data and technical help.
Spectroscopy
High quality spectroscopic analysis with SpectraMax-384 instrument is also available in the facility. Very low volume of samples needed for analysis on this machine. You can analyze 96 samples at a time both for UV and Visible range wavelength .
Oligo/Primer Synthesis
In the past, DNA CORE FACILITY also offered the synthesis of oligonucleotides. These oligonucleotides (oligos) were suitable for PCR, sequencing, site directed mutagenesis and other similar procedures. These oligos/primers were very cheaper as compared to the imported primers. Quality of the products was also up to the international standards. We have high throughput ABI-3900 DNA synthesizer (not functional now) and a team of experienced biochemists.
Note: The facility of DNA Primer Synthesis is not functional now due to technical reasons.
Contact Information:
DR. MUHAMMAD FAROOQ SABAR
Incharge DNA Core Facility
Email: farooqsabar@yahoo.com
Phone: 042-35293141-6 Ext. 117
2) Forensic DNA Typing
3) Molecular Based Diagnostics
4) Plant Biotechnology
Plant Biotechnology & Molecular Biology Lab CAMB, University of the Punjab, Lahore
The Plant Biotechnology and Molecular biology Lab, CAMB University of the Punjab, is engaged with R&D activities ranging from lower plants up to flowering plants. This research group of Plant molecular biologists works on different aspects of molecular basis of Plants. The researchers have wonderful command on differ molecular biology techniques like Gene cloning, expression in Prokaryotic as well as Eukaryote hosts like yeast and plants. The Plant Molecular Biology group possesses the specialties and molecular skills like Plasmid or vector construction, Production of Transgenic plants, Recombinant antigen production and purification of Recombinant Enzymes.
Achievements:
Transgenic sugarcane resistant to sugarcane mosaic virus (SCMV).
Biofertilizers (Identified and characterized PSB microbes).
Molecular identification or DNA Barcoding of Flowering plants, Algae and other microbes.
Services Offered:
CAMB Plant Biotechnology and Molecular Biology group happily offer its services and research facilities to the researchers, university post graduate students and the relevant industry for training and skill development in the areas of:
DNA Barcoding.
Plasmid or vector Construction.
Production of Transgenic plants.
Recombinant antigen production.
Purification of Enzymes from natural and Recombinant sources.
5) Industrial Enzymology
Production of microbial enzymes is an indispensable event in the industrial sectors, due to the high and greater performances of enzymes from different microbes, which works well under a wide range of diverse physical and chemical conditions. Microbial enzymes provide as a potential replacement, in absence or insufficiency of human enzymes. Further, microbial enzymes are the ideal source of industrial enzymes as they can be produced in large quantities in a short period of time and have shorter creation times, and genetic manipulations can be performed more easily on bacterial cells to increase the enzyme production. Still the industries are looking for new microbial strains in order to produce different enzymes to fulfill the enzyme requirements. The researchers have a wonderful command and experience in molecular/ micro/cell biology and virology. The group has expertise in cloning, vector construction and expression studies, Purification of proteins through HPLC and FPLC. PI, Immuno blotting and SSR marker studies in plants.

 

Health Sciences