EDUCATIONAL QUALIFICATION:
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M. Sc Botany (June 1985), Specialization: Microbiology, Government College Lahore, and University, of the Punjab, Lahore, Pakistan.
B. Sc. Biology, (1981), (Botany, Zoology Chemistry), Lahore College for Women, University of the Punjab, Lahore Pakistan.
2005 to present, Assistant Professor in Botany Dept University of the Punjab, Lahore-53700, Pakistan.
2001, 2005, Lecturer in Botany Dept University of the Punjab, Lahore-53700, Pakistan.
1986 to 2001 Lecturer, Center of Excellence in Molecular Biology, University of the Punjab, Lahore-53700, Pakistan.
1985 to 1986, Research Fellow, Center of Excellence in Molecular Biology, University of the Punjab, Lahore 53700, Pakistan.
Role of Honor, 1985
Secondary School Certificate Scholarship.
- Taught basic microbial techniques to M. Phil students.
- Trained the persons from industries about microbial techniques.
- Teaching of B Sc. Honors class
MS. thesis research work was “Microbial Examination of unbottled Beverages”
M. Sc thesis research work included various microbial techniques such as Isolation, identification and purification of bacterial strains from unbotelled beverages. The work also included the quantitative analysis of bacteria.
After completing my M. Sc, I joined the center for advanced Molecular Biology University of the Punjab, Lahore in 1985, started my work on the isolation and characterization of bacteria from local habitat under the supervision of Dr. S. Riazuddin. Firstly I was involved in the isolation of hydrocarbon degrading, sulfur degrading and iron leaching ( Thiobacillaus ferrioxidans ) bacteria. The identification of these bacteria includes morphological and biochemical tests.. I started the project of CAMB culture collection, which are the depository of local bacteria, fungus and yeast as well as some strain of known standard bacteria from abroad. The project work included the isolation, characterization and maintenance for their preservation. All the microbiological techniques are involved for the completion of this work.
I have also learned the basic molecular biological techniques including the transformation, isolation, of DNA, gel electrophoresis, enzyme assays, restriction mapping, lambda DNA preparation etc.
I established the microbial culture collection repository and collected more than one thousand bacterial strains. Along with the inchargeship of culture collection lab I identified, characterized and purified more than one thousand Bacillus thuringinensis strain from local habitat. Which is toxic against certain lepidopteran insects of cotton e.g. spotted ballworm, pink ballworm and American ballworm. All identification process included the protein isolation, purification, western and Dot blot, nature and denature gel electrophoresis and ELISA techniques.
In this connection I have isolated more than 1000 Bt. Isolate from different ecological habitats of Pakistan.
After that I was involved in the production of Polyclonal antibodies against toxic protein, (Cry IAc, Cry, II a, GNA Protein). In this connection I was using the sophisticated technique of SDS-PAGE, ELISA, Western and dot blot.
For the production of antigens I have prepared these protein by electroelution and column chromatography. After the complete characterization and confirmation of purity by western blot this antigen is ready for immunization in rabbits.
Cardiac puncture of rabbit is done to get the whole blood sera for the isolation of antibodies. These antibodies are being used for the immunochemical characterization of transgenic plants of rice and cotton.
Presently I am teaching Microbiology and Phycology to M.Sc and B Sc. Honors in Botany Deptt, University of the Punjab. I am also enrolled with Prof. Dr. Shahida Husnain for my Ph.D studies. The topic of my synopsis is “ Characterization of pesticides degrading Bacteria from local habitats”.My reseach work also include the research supervision of M.Sc students.
