University of the Punjab.

Plant Genomics Lab
Dr. Sameera Hassan has joined CEMB, after Masters in Zoology by selection through national competition, in 1997. During her research endeavours she has worked on phage display of Bacillus thuringiensis protein where the expression of whole fragment of Cry 1 Ac toxin and domains II III were separately displayed as translational fusion with gene III in pDAN3 phagemid system. This study involved different Molecular Biological Techniques like cloning, sub-cloning, SDS PAGE analysis, Dot Blot, Western Blotting, PCR, Plasmid and Genomic DNA Isolation, Colony hybridization, DNA Sequencing and analysis through Chromas software.
Different Microbiological techniques included Streaking, colony inoculum, Glycerol stock and different media preparations.
She has been also an active researcher in Biopharmaceutical lab and worked on the expression of recombinant human erythropoietin (hEPO) gene in prokaryote by transformation in E. coli (BL21DE3) and in eukaryote by transient and stable transfection in Chinese Hamster Ovary cells. The protein was purified by using different chromatographic techniques like Affinity and ion Exchange Chromatography from cell culture supernatants.
She has also worked on multi drug resistant Staphylococcus aureus (MRDSA) from Bovine mastitis dairy samples and isolated 8MRDSA after screening through different microbiological media like Muller Hinton agar, Sheep Blood agar and Baird Parker agar.
In 2010 got enrolled in MPhil leading to PhD program. During MPhil research two desi cotton varieties were worked on for their NaCl tolerance. In 2016 her PhD was notified based on research entitled “Cellular Characterization, Cloning & Expression of Universal Stress Protein Gene (GUSP1) in Cotton and its Role in Drought Tolerance”. Since then, she is working on abiotic/biotic stress tolerant in different plants. The genes playing role in tolerance are isolated, Cloned and characterized for developing transgenic cotton plants for their respective tolerance.
Thus, two PhDs and 15 MPhils have completed their thesis, so far. A total of 10 full length genes and 8 partial cDNAs are submitted on NCBI during 2022-2023.
She is enthusiastic researcher her greatest strength is research and teaching skill which enables her to contribute permanently betterment of her institute as well as to enhance her professional skill.