Estb. 1882

University of the Punjab

Departmental Research

ICARDA’s PAK-US Cotton Productivity Enhancement Project (2010-17)
Virology and Whitefly Haplotyping Component 1198-9
Principal Investigator: Prof. Dr. Muhammad Saleem Haider
Post-Doctoral Research Associate: Dr. M. Zia-Ur-Rehman
Total Project Cost: 500000 USD
Begomoviruses are emerged as a major pathogen of the cotton and other crops in Pakistan particularly in the Punjab region. In past few decades cotton leaf curl disease (CLCuD) caused by Cotton leaf Curl Virus (CLCuV) has caused notable decrease in the cotton yield which resulted in major losses to the Pakistan economy. The disease is spreading in other countries and is also reported in India. CLCuD is a potential threat to all cotton growing countries including USA.
Cotton is the most important fibre crop in the world, as well as the second most important oilseed crop. On a global basis Pakistan is the fourth largest cotton producing country of the world after China, India and USA. Pakistan's share of total world cotton production in 2004/2005 stood at 9.47 percent. All efforts to increase cotton production in Pakistan did not become fruitful because of large losses due to CLCuD. For the last two decades cotton leaf curl disease (CLCuD) has seriously hampered the cultivation and production of cotton crop in Pakistan. Cotton leaf curl disease (CLCuD) caused over US$ 5 billion losses to the Pakistan economy during the mid to late 1990s. The threat of CLCuD is spreading and now it is also a major constraint to cotton production in India. There is a continuous threat that this disease can spread to other countries including USA and can cause serious damages to cotton production. Therefore it is a compelling need to get solution to this problem. This project will help to screen the resistant and susceptible varieties against CLCuD and to generate resistant cotton varieties before the possible outbreak of disease.
The research deals mainly in the screening of cotton germplasm from USA in cotton growing regions of Punjab against CLCuD, determining the symptom severity and characterizing the virus/satellite populations in these lines. Virus diversity in all cultivated and non-cultivated plants from cotton zone will be carried out which will be helpful in devising techniques to generate resistant cotton against wide range of begomoviruses. Haplotyping of whiteflies from this region will provide knowledge about biotype of vector responsible for transmission of this disease.
Here are the major objectives of the study.
Development of elite cotton varieties and hybrids using conventional (intra- and inter-specific) breeding programs with tolerance/resistance to Cotton leaf curl virus (CLCV)-satellite complexes, and stress associated with whitefly vector colonization.
To monitor virus-satellite diversity and viral load (qPCR) in cotton germplasm trials, in relation to susceptible, and resistant or tolerant.
To monitor viral diversity and population structure in ‘sentinel plots' comprising diverse viral hosts, including cultivated and uncultivated species.
To determine whitefly vector haplotype distribution and their associated natural enemies (promising for biological control integrated with other compatible agronomic practices) in and near cotton germplasm plots, and cotton-vegetable cropping systems for designated places.
The study of virus and whitefly population has been completed and cotton resistant against viruses has been developed in collaboration with CEMB scientists and showing promising results during trials in green house. Tomato plants resistant to viruses and whiteflies have been developed in collaboration with University of Arizona scientists and now under trials for resistance screening.
Capacity Development funded by the project
Prof. Dr. M. Saleem Haider and Dr. M. Zia-Ur-Rehman presented their papers in Plant and Animal Genome Conference (PAG XXIII) held in San Diego, CA, USA, January 10-14, 2015.
Dr. Usman Hameed presented his paper in International Cooton Genome Initiative Research Conference 2014 held in China.
Mr. Waheed Anwar and Mr. Javed Iqbal attended American Phytopathology Meeting, 9-13 Augsut, 2014 held in Minneapolis, USA.
Four researchers Dr. Muhammad Zia-Ur-Rehman, Dr. Usman Hameed, Dr. Muhammad Shafiq and Miss Ayesha Bibi have completed advance training from USA, under the Borlaug fellowship program.
Dr. Muhammad Zia-Ur-Rehman was awarded fellowship to complete six month advanced training on use of bioinformatics tools for analyses of genomic data.
Glimpses of Project activities
Quarterly Review Meeting of ICARDA's Pak-US cotton project for the period Jan-Mar, 2013
Meeting was being convened in the Conference Room of Institute of Agricultural Sciences (IAGS),
Punjab University , Lahore on Monday, 25th March 2013. Dr.M.Saleem Haider, PI/Director, IAGS, Lahore hosted and Dr.Brian Scheffler, PI, USDA and Dr.Abdul Majid, Project Mananger, ICARDA chaired the session. All the 12 collaborators, from different research organizations, along with their research groups attended the meeting and shared the updates of the project.
Dr.Brian Scheffler and Ian C. Winborne (USDA official) visited different labs and research field of IAGS on 26th March 2013 and had an introductory meeting with IAGS faculty on 27th March 2013. They briefed the faculty and research students about different scholarship programs offered by US to the Pakistani students and faculty members. They encouraged the faculty members and students to apply for full bright scholarship.
Dr. Brian Scheffler and other USDA officials visiting IAGS research fields
Participants of quarterly meeting at IAGS
Prof. Dr. Judith K. Brown, Prof. Dr. M.Saleem Haider and Dr. Zia-Ur-Rehman Visiting cotton sentinel plots
Cotton leaf curl disease diagnostic and resistance, a practical approach, three days' workshop
Cotton leaf curl disease diagnostic and resistance, a practical approach, three days' workshop was organized by Institute of agricultural sciences, University of the Punjab from 26th to 28th August 2013. Thirty seven participants from all over Pakistan came to attend the workshop and they are from government and private research organization, universities and farmer. On first day of the workshop Inauguration session was chaired by Mr. Armaghan Subhani, member national assembly. Programme began by recitation of verses from the Holy Quran. After that director Institute of Agricultural Sciences and chief organizer of workshop Prof. Dr M. Saleem Haider gave his welcome speech and he highlighted the aims and objects of the workshop. After welcome speech by Dr. Saleem Haider, chief guest Mr. Armaghan Subhani (MNA) addressed workshop participants and expressed that government of Pakistan is very keen to facilitate all research organization working on CLCuV.
Prof. Dr. M. Saleem Haider delivering his talk on virus resistance
A hand on training session on virus diagnosis
International conference on Stress Biology & Biotechnology: Challenges & Management
International conference on Stress Biology & Biotechnology: Challenges & Management was held at IAGS from 21-23 May, 2014. The conference was warmly received by national and international scientists. Around 150 participants including faculty members, researchers and students from Pakistan and abroad participated in the conference. After scrutiny 120 abstracts (70 oral, 50 posters) were accepted for the conference.
Dr. Rob W. Briddon presenting research paper
Dr. Judith K. Brown (University of Arizona) presenting research paper
Prof. Dr. Mounir Abou Haider (University of Toronto, Canada) presenting shield to poster presentation winner
Worthy Vice Chancellor, Prof. Dr. Mujahid Kamran with distinguished guests and participants on opening ceremony of the conference
4th quarterly review and planning meeting of the fiscal year (2014-15)
Fourth quarterly review and planning meeting of the fiscal year (2014-15) of ICARDA's Pak-US cotton project was held at Conference Hall of “Institute of Agricultural Sciences, (IAGS), University of the Punjab, Lahore on Friday, 9th October, 2015. Dr. Noor Ul Islam (Chairman PARB) chaired the meeting and Prof. Dr. M. Saleem Haider hosted the event.
Prof. Dr. M. Saleem Haider handing over the transgenic seed, Prof. Dr. Javed Iqbal Qazi, Dean Faculty of Life Sciences and Dr. Noor-ul-Islam, Chairman PARB are also present
Dr. Noor-Ul-Islam (Chairman PARB) presenting his views about project activities
Participants of the Meeting
Novel Approach to Generate Wide Spectrum Resistance to Cotton Begomoviruses Infecting Cotton and Other Cultivated Crops
Principal Investigator: Prof. Dr. Muhammad Saleem Haider
Total Project Cost: Rs. 28.1 million
Begomoviruses are ssDNA whitefly ( Bemisia tabaci ) transmitted Geminiviruses are known to be a major problem in the cotton and other crops in Pakistan and India particularly in the Punjab region. Crops infected with these viruses show a notable decrease in the yield which results in major losses for farmers in particular and for the nation's economy in general. Transcriptional gene silencing (TGS) refers to the stable repression of transcription and mainly affects chromosomal repeats, transposons and transgenic inserts; however, it may also suppress the expression of certain protein-coding genes. In multicellular eukaryotes, TGS can persist through mitotic divisions and also be inherited meiotically, which is especially well documented for plants. By adopting this technique the transgenic plants in the current research have shown resilience when infected with viral attack. PARB CGS Project 127 entitled “ Novel approach to generate wide spectrum resistance to cotton begomoviruses infecting cotton and other cultivated crops ” has been started on June 1st 2009 with the aim to develop transgenic cotton plants with RNAi-based resistance to cotton leaf curl disease (CLCuD) caused by begomoviruses under the supervision of Dr. Muhammad Saleem Haider (Professor and Director Institute of Agricultural Sciences, University of the Punjab).
The total duration of the project was decided four years with the approval for an amount of Rs. 28.1 million, with the involvement of a collaborator from University of Toronto, Canada. Now the project is extended for the upcoming two years (2014-2015). First and second generation of transgenic cotton plants harboring pART27 IGS-IR construct were sown in CEMB/IAGS fields according to recommended biosafety guidelines. Nevertheless, patent has been filed in Higher Education Commission, Islamabad and for bio-safety clearance National biosafety commission, Islamabad has been intimated regarding certification of our product and response in this regard is awaited.
Sr. #
Title of the Project
Principal Investigator
Cost/Funded by
Effect of genetically modified crops on soil micro-organism and animals. Dr. Ahmad Ali Shahid Rs. 3.5 millions/HEC
Natural compounds from allelopathic trees as antifungal agents against Ascochyta rabiei (Pass.). Dr. Arshad Javid Rs. 2.01 millions/PSF
The control of Jassid on Brinjal through biopesticide and habitat management in Lahore. Dr. Muhammad Ali Rs. 0.5 millions/HEC
Evaluation of weeds extracts in nitrification inhibition and uptake of synthetic nitrogen for yield enhancement of maize. Dr. Rashid Mahmood Rs. 0.5 millions/HEC
Improving the Germinibility and Vigor Enhancement of Sunflower ( Helianthus annuus L.) Hybrid Seed under Varying Temperatures through Seed Priming. Dr. Muhammad Bilal Chattha Rs. 0.5 millions/HEC
Screening of diverse germplasm for drought tolerance in rice ( Oryza sativa L.) through conventional and molecular breeding techniques. Dr. Muhammad Ashfaq Rs. 0.5 millions/HEC
Molecular probing of Mycotoxigenic fungi in stored rice grains in Punjab. Dr. Shinawar Waseem Ali Rs. 0.5 millions/HEC
Sr. no.
Name of principal investigator/designation
Title of research project
Grant recommended (Rs.)
Dr. Muhammad Saleem Haider, Professor
Molecular and biological characterization of begomoviruses and/or associated DNA- satellites from the vicinity of University of the Punjab, Lahore, Pakistan.
Dr. Salik Nawaz Khan, Assistant professor
Host susceptibility against abiotic factors associated with development of stalk rots of maize.
Dr. Ahmed Ali Shahid, Assistant Professor
Use of biological and chemical materials for the control of Fusarium wilt of tomatoes.
Dr. Arshad Javaid, Assistant Professor
Management of Charcoal rot of mungbean by two Tricoderma species and dry biomass of coronopus didymus.
Dr. Shakeel Ahmed, Assistant Professor
In vitro characterization of Anti-oxidative properties of citrus peel extracts against some pathogens of plant.
Dr. Tehmina anjum, Assistant Professor
Management of Bacterial wilt of tomato by using native PGPR bacterial strains.
Dr. Amna Shoaib, Assistant Professor
Effect of chromium on growth and physiology of lycopersicum esculentum Fusarium wilt.
Dr. Uzma Bashir, Assistant Professor
Phenotypic, biochemical and physiological characterization of bacterial strains.
Dr. Shazia Shafique, Assistant Professor
Defense response of selected Eucalyptus species against black spot pathogen of Pisum sativum .
Dr. Muhammad Ashfaq, Assistant Professor
Breeding for the development of rice grain discoloration homozygous resistant population.
Mr. Hameed Ullah malik, Assistant Professor
Determination of the native and exotic birds of the University Quaid-e-Azam campus and the factors affecting their migration.
Dr. Shinawar Waseem ali, Assistant Professor
Analysis of pesticide residues on vegetables being sold in local markets of Lahore city.
Dr. Rashid Mahmood Assistant Professor
Influence of type and combination of phosphorus and potassium fertilizer on soil urease activity.
Malik Fiaz Hussain Ferdosi, Assistant Professor
Effect of electromagnetic radiations to enhance seed germination, seedling vigor and to exploit enzyme activity in Salvia splendens .
Dr. Muhammad Bilal Chattha, Assistant Professor
Physio-agronomic response of spring maize (Zea mays L.) to foliar application of plant growth promoting substances.
Dr. Muhammad Nasir Subhani, Assistant Professor
Bioefficacy of fungicides, bio-chemical agents and botanicals against anthracnose of Chilies incited by Colletotricum capsici .
Dr. Shahbaz Ahmed, Assistant Professor
Integrated pest management in vegetable crops.
Dr. Sobiya Shafique, Assistant Professor
Protective potential of Alstonia Scholaris for the management of charcoal rot of Vigna unguiculata L. Walp.
Dr. Asad Shabbir, Assistant Professor
Impact assessment of parthenium weed on native plant community biodiversity.
Dr. Naureen Akhtar, Assistant Professor
Polyphasic taxonomy of Aspergillus flavus group.
Dr. Maliha Uroos, Assistant Professor
Biological evaluation of natural coumrins and their synthetic analogues.
Dr. Sajid Ali, Assistant Professor
Nitrogen and potash influence the concentration of polyphenols in Black seed ( Nigella sativa L.)
Dr. Nasir Ahmed Assistant Professor
Cloning and Characterization of a Thermostable Starch De-branching Enzyme.
Dr. Zaheer Hussain Assistant Professor
Validation of Flurescent antibody test RT-PCR and Mice Inoculation Test for Diagnosis of Rabies in Pakistan.
Dr. Muhammad Ali Assistant Professor
The integrated pest management (IPM) of Jassid (Amrasca biguttula (Ishida) by the biopesticides on the Okra (Abelmoschus esculentus L.) Monech.
Dr. Muhammad Naeem Sattar, Assistant Professor
Cross-protection study of cotton leaf curl disease associated begomovirus and their associated DNA-satellites by arbuscular mycorrhizal fungi in cotton.
Ms. Zill-e-Huma, Lecturer
Comparative studies on purification of laccases isolated from mushrooms collected from different areas of Lahore.
Ms. Sana Hanif, Lecturer
Enhancing resistance of Rice against bacterial Leaf blight and Rice Blast by flurescent pseudomonads and bacillus Sp.
Mr. Hafiz Azhar Ali Khan, Lecturer
Cross-resistance, genetic and mechanism of resistance to deltamethrin in flies, Musca domestca L., (Diptera:Musidae).
Ms. Qurant-ul-Ain Farooq, Lecturer
A comperative study on germination and early stand establishment of French marigold (Tegetes patula) and African Marigold (Tagetes erecta) seeds by inducing physiological and chemical changes through seed priming.
Mr. Muhammad Shafiq, Lecturer
Virus induced gene silencing based genetic screening for the identification of gene involved in geminivirus resistance.
Mrs. Qurat-ul-Ain Riaz, Lecturer
Seasonal variation in the occurrence of pesticide residues in informal milk marketing chains of Lahore.
Mr. Adnan Zahid, Lecturer
Effect of exogeneous application of Glycinebetaine and proline on the Vigna radiate (L.) under salinity and water deficient conditions.
Mr. Moazzam Anees, Lecturer
Effect of salinity on the vegetative growth behavior of citrus cv. Kinnow mandarin (Citrus reticulate blanco).
Ms. Mehreen Hassan, Lecturer
Effect of varying pot sizes and PGPRs on the growth of selected forest nursery plants.

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